 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Acute and Chronic Gamma Irradiations on In vitro Culture of Anubias congensis N.E. Brown) ผู้เขียน: ปกรณ์ ตั้งปอง, Thanya Taychasinpitak,  ดร.ชูศักดิ์ จอมพุก, รองศาสตราจารย์ , ดร.พีรนุช จอมพุก, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractPlantlets of Anubias congensis N.E. Brown cultured on MS medium were irradiated with gamma rays for both acute and chronic treatments of 0, 20, 40, 60, 80 and 100 Gy at a dose rate of 264 Gy/hr (acute) and 0, 14.34, 28.60, 31.24, 42.90, 51.16, 65.55, 82.42, 91.69, 105.99 and 120.30 Gy at a dose rate of 0.71 Gy/hr (chronic). The calculated LD50(60) for the acute irradiation was 32.12 Gy. It was not possible to calculate the LD50(60) for the chronic irradiation because the survival rate was 100% for all treatments. Following acute gamma irradiation, the number of roots, number of shoots, number of leaves, length and width of leaves and length of roots in the M1V1 generation of A. congensis N.E. Brown plantlets were less than the control. With chronic gamma irradiation, there was no difference in most parameters with the exception of the 105.99 and 120.30 Gy treatments, in which the number of roots was greater than the control. Some abnormalities observed in the M1V1 generation exposed to acute irradiation that were genetically transferred to the M1V3 generation included narrow leaves, undulate leaf margins, and dwarfism with light green leaves. The mutation frequencies for these characters were 11.67, 26.67 and 6.67% for a dose of 20, 40 and 60 Gy, respectively. For the samples exposed to chronic irradiation, mutations observed in the M1V3 generation were dwarfism and albinism. The mutation frequencies were 1.67, 1.67, 3.33, 5.00, 11.67 and 10.00% at a treatment dose of 14.34, 28.60, 31.24, 91.69, 105.99 and 120.30 Gy, respectively. No mutation could be detected in the M1V3 generation irradiation with chronic gamma rays at 42.90, 51.16, 65.55 and 82.42 Gy. The potential mutants of A. congensis N.E. Brown in this research were the undulate leaf margins and dwarfism with light green leaves. All the mutation characters were maintained through tissue culture. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Development and Maintenance of Gynoecious Lines of Cucumber (Cucumis sativus L.)) ผู้เขียน:Badri NARAYAN CHAUDHARY, ดร.เกษม พิลึก, รองศาสตราจารย์ , Thanya Taychasinpitak, นายชัยฤกษ์ สงวนทรัพยากร, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe study aimed to develop gynoecious lines of cucumber (Cucumis sativus L.) by isolating, selfing and evaluating of selfed progenies from original populations and to maintain these lines by using chemicals to induce staminate flower for genetically selfing. Two F1 cucumber cultivars of long type (Seminis-1 and Seminis-2) and three short type (Siminis-3, Micro-c and Bingo) from Thailand and three OP cultivars of long type (Long Green, Kusle and Bhakatpur Local) from Nepal were evaluated for gynoecious sex expression. Among the F1 populations, only Bingo expressed gynoecious type for 5% and the rest were predominantly gynoecious sex type. Open-pollinated populations only expressed monoecious sex type. During the process of gynoecious line development through inbreeding and plant-to-row selection it took three consecutive selfing generation (S3) for complete gynoecious development of SE1-G (long) and SE3- G (short) lines which were isolated from the original population of Seminis-1 and Seminis-3 respectively. Among the used chemical, silver nitrate (AgNo3) was found statistically significantly superior over gibberellic acid (GA3) and silver thiosulfate (Ag(S2O2)2) for effective staminate flower induction for the maintenance of gynoecious lines. The highest sex ratio (M/F) 0.80:1 in SE1-G line and 0.89:1 in SE3-G line was observed by first lateral chemical application from the chemical silver nitrate 400 and 300 ppm applied twice respectively which confirm the highest possibility of flower synchronization. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Acute Gamma Irradiation on Adventitious Plantlet Regeneration and Mutation from Leaf Cuttings of African Violet (Saintpaulia ionantha)) ผู้เขียน:นางอรุณี วงศ์ปิยะสถิตย์, ศาสตราจารย์, Tanita Thinnok, Thanya Taychasinpitak, ดร.พีรนุช จอมพุก, รองศาสตราจารย์, ดร.คทารัตน์ ชุตินันทกุล, ผู้ช่วยศาสตราจารย์ , นางสิรนุช ลามศรีจันทร์, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractLeaf cuttings of Saintpaulia ionantha (African violet) cv. Optima Hawaii, purple flower were acutely irradiated by gamma-rays with the doses of 0, 10, 20, 40, 60, 80 and 100 gray (Gy) to induce mutation. Irradiated leaves (M1V0) were planted in plastic trays containing peat moss medium and were placed in a shaded greenhouse. A number of adventitious plantlets per irradiated leaf (M1V1/M1V0) were recorded. M1V1 plantlets were transplanted and were grown to flowering according to the standard African violet culture. The characters investigated in M1V1 plants were the number of leaves per plant, plant canopy width, the number of inflorescences per plant, the number of flowers per inflorescence, flower size and mutated characters. The results showed LD50(60) to be 56 Gy. The number of plantlets per leaf decreased as radiation doses increased. No survived leaves were observed at doses higher than 80 Gy. In M1V1 generation, averaged width of plant canopy and the number of flowers per inflorescence were not significantly different whereas the number of inflorescences per M1V1 plant and flower size were significantly different. Mutation characters observed were – changes in leaf colour, leaf margin, leaf thickness, flower colour, flower size, flower form and plant type. Mutation rate of M1V1 plants increased as radiation doses increased. Seven mutants with desirable characters were selected. |